CDH1 Antibody

Mouse Monoclonal Antibody (Mab)

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AM2190b staining CDH1 in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
All lanes : Anti-CDH1 at 1:4000 dilution Lane 1: SW480 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: WiDr whole cell lysate Lane 4: Mouse stomach lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 98 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

产品详情
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实验流程
背景知识

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	P12830
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Isotype	IgG1,k
Clone Names	813CT11.1.3
Calculated MW	97456 Da

Additional Information
Gene ID	999
Other Names	Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324, E-Cad/CTF1, E-Cad/CTF2, E-Cad/CTF3, CDH1, CDHE, UVO
Target/Specificity	Purified His-tagged CDH1 protein was used to produced this monoclonal antibody.
Dilution	WB~~1:4000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CDH1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CDH1 (HGNC:1748)
Function	Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed:29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.
Cellular Location	Cell junction, adherens junction. Cell membrane; Single-pass type I membrane protein Endosome. Golgi apparatus, trans-Golgi network. Cytoplasm. Cell junction, desmosome. Note=Colocalizes with DLGAP5 at sites of cell-cell contact in intestinal epithelial cells. Anchored to actin microfilaments through association with alpha-, beta- and gamma- catenin. Sequential proteolysis induced by apoptosis or calcium influx, results in translocation from sites of cell-cell contact to the cytoplasm. Colocalizes with RAB11A endosomes during its transport from the Golgi apparatus to the plasma membrane. Recruited to desmosomes at the initial assembly phase and also accumulates progressively at mature desmosome cell-cell junctions (PubMed:25208567, PubMed:29999492) Localizes to cell-cell contacts as keratinocyte differentiation progresses (By similarity). {ECO:0000250\|UniProtKB:P09803, ECO:0000269\|PubMed:25208567, ECO:0000269\|PubMed:29999492}
Tissue Location	Expressed in granuloma macrophages (at protein level) (PubMed:27760340). Expressed in the skin (at protein level) (PubMed:22294297). Expressed in the liver (PubMed:3263290)

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

REFERENCES

Bussemakers M.J.G., et al. Mol. Biol. Rep. 17:123-128(1993).
Oda T., et al. Proc. Natl. Acad. Sci. U.S.A. 91:1858-1862(1994).
Rimm D.L., et al. Biochem. Biophys. Res. Commun. 200:1754-1761(1994).
Ito K., et al. Oncogene 18:7080-7090(1999).
Bussemakers M.J.G., et al. Biochem. Biophys. Res. Commun. 203:1284-1290(1994).

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任勇刚 2016-04-12 12:06:29 1 2 3 4 5	Species tested Human Application tested WB Organization tested 肝癌细胞 Barcode encoding Brief protocol
任勇刚 2016-04-12 12:05:52 1 2 3 4 5	Species tested Human Application tested IHC Organization tested 肝癌细胞，肝癌组织 Barcode encoding Brief protocol IHC

¥ 1,250.00

Cat# AM2190b

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¥ 2,960.00

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CDH1 Antibody

Mouse Monoclonal Antibody (Mab)

BACKGROUND

REFERENCES

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