CHK1 Antibody 精选

Mouse Monoclonal Antibody (Mab)

All lanes : Anti-CHK1 Antibody at 1:1000-1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lane 5: MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgM, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
The anti-CHK1 Mab (Cat. #AM7401a) is used in Western blot to detect CHK1 in NIH/3T3 cell lysate (Lane 1) and K562 cell lysate (Lane 2).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Primary Accession	O14757
Reactivity	Human, Mouse
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgM
Clone Names	2G1D5

Additional Information
Other Names	Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1, CHK1
Target/Specificity	This monoclonal antibody is generated from mice immunized with Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15.
Dilution	WB~~1:1,000~4,000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CHK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.