Tlr2 Antibody

Purified Mouse Monoclonal Antibody

Figure 1:Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
Figure 2:Western blot analysis using Tlr2 mAb against mouse Tlr2 (AA: 628-777) recombinant protein. (Expected MW is 44 kDa)
Figure 3:Western blot analysis using Tlr2 mAb against HEK293 (1) and Tlr2 (AA: 628-777)-hIgGFc transfected HEK293 (2) cell lysate.
Figure 4:Immunofluorescence analysis of HepG2 cells using Tlr2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ICC, E
Primary Accession	Q9QUN7
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Clone Names	7G5F4
Isotype	IgG1
Calculated MW	89449 Da
Description	the activation of toll-like receptor (TLR) 2 induces oxidative stress and inflammation, TLR2 may be directly linked to skeletal muscle atrophy.
Immunogen	Purified recombinant fragment of mouse Tlr2 (AA: 628-777) expressed in E. Coli.
Formulation	Purified antibody in PBS with 0.05% sodium azide

Additional Information
Other Names	Toll-like receptor 2, CD282, Tlr2
Dilution	WB~~1/500 - 1/2000 ICC~~N/A E~~1/10000
Storage	Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	Tlr2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.