CHCHD2 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

CHCHD2 Antibody (Center) (Cat. #AP10055c) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CHCHD2 antibody detected the CHCHD2 protein (arrow).
CHCHD2 antibody (Center) (Cat. #AP10055c) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHCHD2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
CHCHD2 Antibody (Center) (Cat. #AP10055c) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	Q9Y6H1
Other Accession	NP_057223.1
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	15513 Da
Antigen Region	74-103 aa

Additional Information
Gene ID	51142
Other Names	Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, mitochondrial, Aging-associated gene 10 protein, HCV NS2 trans-regulated protein, NS2TP, CHCHD2, C7orf17
Target/Specificity	This CHCHD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-103 amino acids from the Central region of human CHCHD2.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	CHCHD2 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	CHCHD2
Synonyms	C7orf17
Function	Transcription factor. Binds to the oxygen responsive element of COX4I2 and activates its transcription under hypoxia conditions (4% oxygen), as well as normoxia conditions (20% oxygen) (PubMed:23303788).
Cellular Location	Nucleus. Mitochondrion. Mitochondrion intermembrane space. Note=Mainly localized in the intermembrane space.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.