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>   首页   >   产品   >   一抗   >   信号转导   >   M Mlkl Antibody (C-term)   

M Mlkl Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - M Mlkl Antibody (C-term) AP14272B
    All lanes : Anti-Mlkl Antibody (C-term) at 1:2000 dilution Lane 1: mouse lung lysates Lane 2: NIH/3T3 whole cell lysates Lane 3: mouse liver lysates Lane 4: mouse testis whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 54 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9D2Y4
Other Accession NP_083281.1
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 54317 Da
Additional Information
Gene ID 74568
Other Names Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818}
Target/Specificity This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsM Mlkl Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Mlkl {ECO:0000303|PubMed:23835476, ECO:0000312|MGI:MGI:1921818}
Function Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:23835476, PubMed:27321907, PubMed:24012422, PubMed:24019532, PubMed:32200799, PubMed:32296175). Does not have protein kinase activity (PubMed:24012422). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:23835476, PubMed:27321907, PubMed:24012422, PubMed:24019532). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed:32200799, PubMed:32296175). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (By similarity).
Cellular Location Cytoplasm. Cell membrane. Nucleus. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (By similarity). Localizes to the nucleus in response to orthomyxoviruses infection (PubMed:32200799). {ECO:0000250|UniProtKB:Q8NB16, ECO:0000269|PubMed:32200799}
Tissue Location Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain
Research Areas

BACKGROUND

The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.

REFERENCES

Bisson, N., et al. Cell Cycle 7(7):909-916(2008)

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