M Mlkl Antibody (C-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 文献引用 : 32
- 实验流程
- 背景知识
Application ![]()
| WB, IHC-P, E |
---|---|
Primary Accession | Q9D2Y4 |
Other Accession | NP_083281.1 |
Reactivity | Mouse |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 54317 Da |
Gene ID | 74568 |
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Other Names | Mixed lineage kinase domain-like protein, Mlkl {ECO:0000312|EMBL:AAH237551, ECO:0000312|MGI:MGI:1921818} |
Target/Specificity | This Mouse Mlkl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 444-472 amino acids from the C-terminal region of mouse Mlkl. |
Dilution | WB~~1:2000 |
Format | Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Precautions | M Mlkl Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Mlkl {ECO:0000303|PubMed:23835476, ECO:0000312|MGI:MGI:1921818} |
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Function | Pseudokinase that plays a key role in TNF-induced necroptosis, a programmed cell death process (PubMed:23835476, PubMed:27321907, PubMed:24012422, PubMed:24019532, PubMed:32200799, PubMed:32296175). Does not have protein kinase activity (PubMed:24012422). Activated following phosphorylation by RIPK3, leading to homotrimerization, localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage (PubMed:23835476, PubMed:27321907, PubMed:24012422, PubMed:24019532). In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol (PubMed:32200799, PubMed:32296175). Binds to highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which is essential for its necroptotic function (By similarity). |
Cellular Location | Cytoplasm. Cell membrane. Nucleus. Note=Localizes to the cytoplasm and translocates to the plasma membrane on necroptosis induction (By similarity). Localizes to the nucleus in response to orthomyxoviruses infection (PubMed:32200799). {ECO:0000250|UniProtKB:Q8NB16, ECO:0000269|PubMed:32200799} |
Tissue Location | Highly expressed in thymus, colon, intestine, liver, spleen and lung. Expressed at much lower level in skeletal muscle, heart and kidney. Not detected in brain |
Research Areas

Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein kinase domain is predicted to be catalytically inactive. Molecular function: protein binding. There are two isoforms.
REFERENCES
Bisson, N., et al. Cell Cycle 7(7):909-916(2008)

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