癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
NEDD9 Antibody (Center)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 实验流程
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Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | Q14511 |
| Other Accession | NP_006394.1, NP_892011.2 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 92861 Da |
| Antigen Region | 335-363 aa |
| Gene ID | 4739 |
|---|---|
| Other Names | Enhancer of filamentation 1, hEF1, CRK-associated substrate-related protein, CAS-L, CasL, Cas scaffolding protein family member 2, Neural precursor cell expressed developmentally down-regulated protein 9, NEDD-9, Renal carcinoma antigen NY-REN-12, p105, Enhancer of filamentation 1 p55, NEDD9, CASL, CASS2 |
| Target/Specificity | This NEDD9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 335-363 amino acids from the Central region of human NEDD9. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | NEDD9 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NEDD9 (HGNC:7733) |
|---|---|
| Function | Scaffolding protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion (PubMed:24574519). As a focal adhesion protein, plays a role in embryonic fibroblast migration (By similarity). May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B- and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRKL and SHPTP2 to the tyrosine phosphorylated form (PubMed:9020138). Promotes adhesion and migration of lymphocytes; as a result required for the correct migration of lymphocytes to the spleen and other secondary lymphoid organs (PubMed:17174122). Plays a role in the organization of T-cell F- actin cortical cytoskeleton and the centralization of T-cell receptor microclusters at the immunological synapse (By similarity). Negatively regulates cilia outgrowth in polarized cysts (By similarity). Modulates cilia disassembly via activation of AURKA-mediated phosphorylation of HDAC6 and subsequent deacetylation of alpha-tubulin (PubMed:17604723). Positively regulates RANKL-induced osteoclastogenesis (By similarity). Required for the maintenance of hippocampal dendritic spines in the dentate gyrus and CA1 regions, thereby involved in spatial learning and memory (By similarity). |
| Cellular Location | Cytoplasm, cell cortex. Nucleus. Golgi apparatus. Cell projection, lamellipodium. Cytoplasm. Cell junction, focal adhesion. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle pole. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium basal body Basolateral cell membrane {ECO:0000250|UniProtKB:A0A8I3PDQ1} |
| Tissue Location | Expressed in B-cells (at protein level) (PubMed:9020138). Expressed in the respiratory epithelium of the main bronchi to the bronchioles in the lungs (at protein level) (PubMed:9584194). High levels detected in kidney, lung, and placenta (PubMed:9584194). Expressed in lymphocytes (PubMed:9497377) |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Docking protein which plays a central coordinating role for tyrosine-kinase-based signaling related to cell adhesion. May function in transmitting growth control signals between focal adhesions at the cell periphery and the mitotic spindle in response to adhesion or growth factor signals initiating cell proliferation. May play an important role in integrin beta-1 or B cell antigen receptor (BCR) mediated signaling in B-and T-cells. Integrin beta-1 stimulation leads to recruitment of various proteins including CRK, NCK and SHPTP2 to the tyrosine phosphorylated form.
REFERENCES
Corneveaux, J.J., et al. Hum. Mol. Genet. 19(16):3295-3301(2010)
Lucas, J.T. Jr., et al. Oncogene 29(31):4449-4459(2010)
Tedde, A., et al. Neurosci. Lett. 477(3):121-123(2010)
Malleter, M., et al. Int. J. Mol. Med. 25(6):897-903(2010)
Laumet, G., et al. J. Alzheimers Dis. 20(4):1181-1188(2010)
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