癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SMO Antibody (Center) 精选
Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 文献引用 : 3
- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q99835 |
| Other Accession | NP_005622.1 |
| Reactivity | Human |
| Predicted | Mouse, Rat, Bovine, Canine, Rabbit, Chicken |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 86397 Da |
| Antigen Region | 539-567 aa |
| Gene ID | 6608 |
|---|---|
| Other Names | Smoothened homolog, SMO, Protein Gx, SMO, SMOH |
| Target/Specificity | This SMO antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 539-567 amino acids from the Central region of human SMO. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SMO Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | SMO {ECO:0000303|PubMed:8906787, ECO:0000312|HGNC:HGNC:11119} |
|---|---|
| Function | G protein-coupled receptor, which transduces the smoothened signaling pathway (PubMed:19592253, PubMed:27437577, PubMed:28344083, PubMed:31168089, PubMed:32929279, PubMed:36202993). Activated by cholesterol in response to hedgehog (DHH, IHH or SHH) morphogens (PubMed:27437577, PubMed:28344083, PubMed:32929279). In absence of hedgehog, SMO is inactivated by patched protein (PTCH1 or PTCH2), which prevents SMO access to cholesterol (PubMed:28344083). In response to hedgehog-binding to pathched, inhibition is relieved, promoting SMO translocation to primary cilium and activation by cholesterol: cholesterol causes a conformation change that triggers signaling via G protein G(i), mediating inhibition of adenylate cyclase activity and decreassed production of cAMP (PubMed:28344083, PubMed:31168089). Decreased cAMP levels then inhibit protein kinase A (PKA) and promote release of GLI (GLI1, GLI2 and GLI3) transcription factors, which activate expression of target genes (PubMed:31168089). Active SMO also directly inhibits PKA by sequestering its catalytic subunit, PRKACA, at the cell membrane, preventing PRKACA-mediated phosphorylation and subsequent processing of GLI transcription factors (PubMed:36202993, PubMed:39138140). Active SMO also promotes the removal of GPR161, a key inhibitor of the smoothened signaling pathway, from primary cilia (By similarity). |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P56726}; Multi-pass membrane protein. Cell projection, cilium membrane; Multi-pass membrane protein. Note=Translocates to primary cilium in response to hedgehog (DHH, IHH or SHH) morphogens (By similarity) Cilium localization is mediated via a beta-arrestin and KIF3A-dependent mechanism (By similarity). {ECO:0000250|UniProtKB:P56726} |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The protein encoded by this gene is a G protein-coupled receptor that interacts with the patched protein, a receptor for hedgehog proteins. The encoded protein tranduces signals to other proteins after activation by a hedgehog protein/patched protein complex.
REFERENCES
Zhang, L., et al. Oral Dis 16(8):818-822(2010)
Desch, P., et al. Oncogene 29(35):4885-4895(2010)
Walter, K., et al. Clin. Cancer Res. 16(6):1781-1789(2010)
Hirotsu, M., et al. Mol. Cancer 9, 5 (2010) :
Rittie, L., et al. Aging Cell 8(6):738-751(2009)
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