RNF166 Antibody (Center) 精选

Affinity Purified Rabbit Polyclonal Antibody (Pab)

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Fluorescent image of NIH/3T3 cells stained with RNF166 Antibody (Center)(Cat#AP20484C). AP20484C was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
RNF166 Antibody (Center) (Cat. #AP20484c) western blot analysis in 293,mouse NIH/3T3 cell line and testis tissue lysates (35ug/lane).This demonstrates the RNF166 antibody detected the RNF166 protein (arrow).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF, E
Primary Accession	Q96A37
Other Accession	Q6J1I7, Q3U9F6
Reactivity	Human, Rat, Mouse
Predicted	Rat, Bovine, Canine, Chicken
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	26122 Da
Antigen Region	71-97 aa

Additional Information
Gene ID	115992
Other Names	RING finger protein 166, RNF166
Target/Specificity	This RNF166 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-97 amino acids from the Central region of human RNF166.
Dilution	WB~~1:1000 IF~~1:25 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	RNF166 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	RNF166
Function	E3 ubiquitin-protein ligase that promotes the ubiquitination of different substrates (PubMed:27880896). In turn, participates in different biological processes including interferon production or autophagy (PubMed:26456228, PubMed:27880896). Plays a role in the activation of RNA virus-induced interferon-beta production by promoting the ubiquitination of TRAF3 and TRAF6 (PubMed:26456228). Also plays a role in the early recruitment of autophagy adapters to bacteria (PubMed:27880896). Mediates 'Lys-29' and 'Lys-33'-linked ubiquitination of SQSTM1 leading to xenophagic targeting of bacteria and inhibition of their replication (PubMed:27880896).
Cellular Location	Cytoplasm.