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Anti-AIOLOS Antibody

Rabbit polyclonal antibody to AIOLOS

     
  • 1 - Anti-AIOLOS Antibody AP60323
    Western blot analysis of AIOLOS expression in HepG2 (A), HeLa (B) whole cell lysates.
  • 3 - Anti-AIOLOS Antibody AP60323
    Immunofluorescent analysis of AIOLOS staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF/IC
Primary Accession Q9UKT9
Reactivity Human, Monkey, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 58023 Da
Additional Information
Gene ID 22806
Other Names ZNFN1A3; Zinc finger protein Aiolos; Ikaros family zinc finger protein 3
Target/Specificity Recognizes endogenous levels of AIOLOS protein.
Dilution WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
IF/IC~~N/A
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name IKZF3
Synonyms ZNFN1A3
Function Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner.
Cellular Location Nucleus. Cytoplasm [Isoform 3]: Nucleus [Isoform 14]: Nucleus. Cytoplasm
Tissue Location Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AIOLOS. The exact sequence is proprietary.

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