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Anti-NSG1 Antibody

Rabbit polyclonal antibody to NSG1

Western blot analysis of NSG1 expression in mouse muscle (A), rat muscle (B) whole cell lysates.
Immunohistochemical analysis of NSG1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P42857
Other Accession	Q62092
Reactivity	Human, Mouse, Rat, Monkey
Host	Rabbit
Clonality	Polyclonal
Calculated MW	20913 Da

Additional Information
Gene ID	27065
Other Names	D4S234; Neuron-specific protein family member 1; Brain neuron cytoplasmic protein 1
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NSG1. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	NSG1 (HGNC:18790)
Function	Plays a role in the recycling mechanism in neurons of multiple receptors, including AMPAR, APP and L1CAM and acts at the level of early endosomes to promote sorting of receptors toward a recycling pathway. Regulates sorting and recycling of GRIA2 through interaction with GRIP1 and then contributes to the regulation of synaptic transmission and plasticity by affecting the recycling and targeting of AMPA receptors to the synapse (By similarity). Is required for faithful sorting of L1CAM to axons by facilitating trafficking from somatodendritic early endosome or the recycling endosome (By similarity). In an other hand, induces apoptosis via the activation of CASP3 in response to DNA damage (PubMed:20599942, PubMed:20878061).
Cellular Location	Membrane {ECO:0000250\|UniProtKB:P02683}; Single- pass type II membrane protein {ECO:0000250\|UniProtKB:P02683}. Golgi apparatus, trans-Golgi network membrane {ECO:0000250\|UniProtKB:P02683} Endosome membrane {ECO:0000250\|UniProtKB:P02683}. Cell projection, dendrite {ECO:0000250\|UniProtKB:P02683}. Early endosome membrane {ECO:0000250\|UniProtKB:P02683}. Late endosome membrane {ECO:0000250\|UniProtKB:P02683}. Lysosome lumen {ECO:0000250\|UniProtKB:P02683}. Recycling endosome membrane {ECO:0000250\|UniProtKB:P02683}. Cytoplasmic vesicle membrane {ECO:0000250\|UniProtKB:P02683}. Golgi apparatus, Golgi stack membrane {ECO:0000250\|UniProtKB:P02683}. Endosome, multivesicular body membrane {ECO:0000250\|UniProtKB:P02683}. Endoplasmic reticulum membrane. Note=Endocytosed from the cell surface, thus enters into early endosomes, trafficks to late endosomes and degradates in lysosomes (By similarity). Endoplasmic reticulum targeting is essential for apoptosis (PubMed:20599942). Found in both stationary and motile endosomes. A previous study supports a type I membrane protein topology (By similarity) {ECO:0000250\|UniProtKB:P02683, ECO:0000250\|UniProtKB:Q62092, ECO:0000269\|PubMed:20599942}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol