Anti-ARHGEF7 Antibody

Rabbit polyclonal antibody to ARHGEF7

Western blot analysis of ARHGEF7 expression in rat kidney (A), rat testis (B) whole cell lysates.
Immunohistochemical analysis of ARHGEF7 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q14155
Other Accession	Q9ES28
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	90012 Da

Additional Information
Gene ID	8874
Other Names	COOL1; KIAA0142; P85SPR; PAK3BP; PIXB; Rho guanine nucleotide exchange factor 7; Beta-Pix; COOL-1; PAK-interacting exchange factor beta; p85
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human ARHGEF7. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200) IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ARHGEF7
Synonyms	COOL1, KIAA0142, P85SPR, PAK3BP, PIXB
Function	Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions (By similarity). May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
Cellular Location	Cell junction, focal adhesion. Cell projection, ruffle. Cytoplasm, cell cortex Cell projection, lamellipodium. Note=Detected at cell adhesions. A small proportion is detected at focal adhesions

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.