Anti-NT5C1A Antibody

Rabbit polyclonal antibody to NT5C1A

Western blot analysis of NT5C1A expression in HCT116 (A), COS7 (B), mouse heart (C), PC12 (D) whole cell lysates.
Immunofluorescent analysis of NT5C1A staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF/IC
Primary Accession	Q9BXI3
Other Accession	A3KFX0
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	41021 Da

Additional Information
Gene ID	84618
Other Names	Cytosolic 5'-nucleotidase 1A; cN1A; Cytosolic 5'-nucleotidase IA; cN-I; cN-IA
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human NT5C1A. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IF/IC (1/100 - 1/500) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	NT5C1A
Function	Catalyzes the hydrolysis of ribonucleotide and deoxyribonucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside (PubMed:11133996, PubMed:34814800, PubMed:7599155, PubMed:8967393). AMP is the major substrate but can also hydrolyze dCMP and IMP (PubMed:11133996, PubMed:34814800, PubMed:7599155, PubMed:8967393).
Cellular Location	Cytoplasm.
Tissue Location	Highly expressed in skeletal muscle. Detected at intermediate levels in heart, brain, kidney and pancreas