Anti-VPS54 Antibody

Rabbit polyclonal antibody to VPS54

Western blot analysis of VPS54 expression in mouse testis (A), rat testis (B), HEK293T (C) whole cell lysates.
Immunohistochemical analysis of VPS54 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	Q9P1Q0
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	110589 Da

Additional Information
Gene ID	51542
Other Names	HCC8; Vacuolar protein sorting-associated protein 54; Hepatocellular carcinoma protein 8; Tumor antigen HOM-HCC-8; Tumor antigen SLP-8p
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human VPS54. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/2000), IHC (1/50 - 1/200) IHC~~WB (1/500 - 1/2000), IHC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	VPS54
Synonyms	HCC8
Function	Acts as a component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD (PubMed:18367545). Within the GARP complex, required to tether the complex to the TGN. Not involved in endocytic recycling (PubMed:25799061).
Cellular Location	Golgi apparatus, trans-Golgi network. Membrane {ECO:0000250\|UniProtKB:Q9JMK8}. Note=Associates with membranes in an EIPR1-independent manner. {ECO:0000250\|UniProtKB:Q9JMK8}

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.