Anti-Synaptotagmin (pT202/199) Antibody

Rabbit polyclonal antibody to Synaptotagmin (pT202/199)

Western blot analysis of Synaptotagmin (pT202/199) expression in K562 (A), HEK293T (B), U87MG (C), A549 (D) whole cell lysates.
Immunohistochemical analysis of Synaptotagmin (pT202/199) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P21579, Q8N9I0
Other Accession	P46096, P46097
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Calculated MW	47573 Da

Additional Information
Gene ID	6857
Other Names	SYT1; SVP65; SYT; Synaptotagmin-1; Synaptotagmin I; SytI; p65; SYT2; Synaptotagmin-2; Synaptotagmin II; SytII
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Synaptotagmin. The exact sequence is proprietary.
Dilution	WB~~WB (1/500 - 1/1000), IHC (1/50 - 1/100) IHC~~WB (1/500 - 1/1000), IHC (1/50 - 1/100)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	SYT1 (HGNC:11509)
Synonyms	SVP65, SYT
Function	Calcium sensor that participates in triggering neurotransmitter release at the synapse (By similarity). May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse (By similarity). It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)- dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes (PubMed:23999003).
Cellular Location	Cytoplasmic vesicle, secretory vesicle membrane {ECO:0000250\|UniProtKB:P21707}; Single-pass membrane protein. Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane {ECO:0000250\|UniProtKB:P21707}; Single-pass membrane protein {ECO:0000250\|UniProtKB:P21707}. Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane {ECO:0000250\|UniProtKB:P21707}; Single-pass membrane protein {ECO:0000250\|UniProtKB:P21707}. Cytoplasm {ECO:0000250\|UniProtKB:P21707}
Tissue Location	Expressed in melanocytes (PubMed:23999003).