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>   首页   >   产品   >   一抗   >   其他   >   Anti-Rhodopsin (pS334) Antibody   

Anti-Rhodopsin (pS334) Antibody

Rabbit polyclonal antibody to Rhodopsin (pS334)

     
  • 1 - Anti-Rhodopsin (pS334) Antibody AP61446
    Western blot analysis of Rhodopsin (pS334) expression in mouse eyes (A) whole cell lysates.
  • 19 - Anti-Rhodopsin (pS334) Antibody AP61446
    Immunohistochemical analysis of Rhodopsin (pS334) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC
Primary Accession P08100
Other Accession P15409
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 38893 Da
Additional Information
Gene ID 6010
Other Names OPN2; Rhodopsin; Opsin-2
Target/Specificity Recognizes endogenous levels of Rhodopsin with a site at pS334 protein.
Dilution WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200)
IHC~~1:100~500
Format Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name RHO
Synonyms OPN2
Function Photoreceptor required for image-forming vision at low light intensity (PubMed:7846071, PubMed:8107847). Required for photoreceptor cell viability after birth (PubMed:12566452, PubMed:2215617). Light- induced isomerization of the chromophore 11-cis-retinal to all-trans- retinal triggers a conformational change that activates signaling via G-proteins (PubMed:26200343, PubMed:28524165, PubMed:28753425, PubMed:8107847). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:26200343, PubMed:28524165).
Cellular Location Membrane; Multi-pass membrane protein. Cell projection, cilium, photoreceptor outer segment. Note=Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to disk membranes in the rod outer segment (OS) photosensory cilia
Tissue Location Rod shaped photoreceptor cells which mediate vision in dim light
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human Rhodopsin with a site at pS334. The exact sequence is proprietary.

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