Anti-Defensin alpha 3 Antibody

Rabbit polyclonal antibody to Defensin alpha 3

Western blot analysis of Defensin alpha 3 expression in H1688 (A) whole cell lysates.
Immunohistochemical analysis of Defensin alpha 3 staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P59666
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Calculated MW	10245 Da

Additional Information
Gene ID	1668
Other Names	DEF3; Neutrophil defensin 3; Defensin alpha 3; HNP-3; HP-3; HP3
Target/Specificity	Recognizes endogenous levels of Defensin alpha 3 protein.
Dilution	WB~~WB (1/500 - 1/1000), IH (1/50 - 1/200) IHC~~1:100~500
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	DEFA3
Synonyms	DEF3
Function	Effector molecule of the innate immune system that acts via antibiotic-like properties against a broad array of infectious agents including bacteria, fungi, and viruses (PubMed:15616305, PubMed:15772169, PubMed:17142766). Possesses the ability to neutralize bacterial toxins such as B.anthracis lethal factor, Clostridium difficile cytotoxin B as well as leukocidin produced by Staphylococcus aureus (PubMed:15772169, PubMed:18435932, PubMed:25963798). Also blocks herpes simplex virus infection by interacting with envelope glycoprotein B and thus preventing its binding to heparan sulfate, the receptor for attachment (PubMed:17142766).
Cellular Location	Secreted.