Anti-MID1 Antibody
Rabbit polyclonal antibody to MID1
- 产品详情
- 实验流程
- 背景知识
Application ![]()
| WB, IF/IC, IHC |
---|---|
Primary Accession | O15344 |
Other Accession | O70583 |
Reactivity | Human, Mouse, Rat, Monkey, Drosophila |
Host | Rabbit |
Clonality | Polyclonal |
Calculated MW | 75251 Da |
Gene ID | 4281 |
---|---|
Other Names | FXY; RNF59; TRIM18; XPRF; E3 ubiquitin-protein ligase Midline-1; Midin; Putative transcription factor XPRF; RING finger protein 59; RING finger protein Midline-1; Tripartite motif-containing protein 18 |
Target/Specificity | Recognizes endogenous levels of MID1 protein. |
Dilution | WB~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500) IF/IC~~N/A IHC~~WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF/IC (1/100 - 1/500) |
Format | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide. |
Storage | Store at -20 °C.Stable for 12 months from date of receipt |
Name | MID1 |
---|---|
Synonyms | FXY, RNF59, TRIM18, XPRF |
Function | Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination. |
Cellular Location | Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Note=Microtubule- associated. It is associated with microtubules throughout the cell cycle, co-localizing with cytoplasmic fibers in interphase and with the mitotic spindle and midbodies during mitosis and cytokinesis |
Tissue Location | In the fetus, highest expression found in kidney, followed by brain and lung. Expressed at low levels in fetal liver. In the adult, most abundant in heart, placenta and brain |
Research Areas
For Research Use Only. Not For Use In Diagnostic Procedures.
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human MID1. The exact sequence is proprietary.

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