Anti-VSV-G-tag Antibody

Mouse monoclonal antibody to VSV-G-tag

Western blot analysis of over-expressed VSV-G-tagged protein in 293T cell lysate.
Immunofluorescent analysis of VSV-G-tag staining in 293T cells transfected with a VSV-G-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunoprecipitation of VSV-G-tagged protein from HEK293T cells transfected with vector overexpressing VSV-G tag, using Anti-VSV-G-tag Antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IP, IF/IC
Host	Mouse
Clonality	Monoclonal

Additional Information
Target/Specificity	Recognizes C-terminal, internal, and N-terminal VSV-G-tag fusion proteins.
Dilution	WB~~WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200) IP~~WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200) IF/IC~~N/A
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.