Anti-Alpha-tubulin (AcK40) Antibody

Mouse monoclonal antibody to Alpha-tubulin (AcK40)

Western blot analysis of Alpha-tubulin (AcK40) expression in HeLa (A) whole cell lysates.
Immunohistochemical analysis of Alpha-tubulin (AcK40) staining in human breast cancer,mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P68363
Reactivity	Human, Mouse, Rat
Host	Mouse
Clonality	Monoclonal
Calculated MW	50152 Da

Additional Information
Gene ID	10376
Other Names	Tubulin alpha-1B chain; Alpha-tubulin ubiquitous; Tubulin K-alpha-1; Tubulin alpha-ubiquitous chain
Target/Specificity	KLH-conjugated synthetic peptide encompassing a sequence of human Alpha-tubulin. The exact sequence is proprietary.
Dilution	WB~~WB (1/1000 - 1/2000), IHC (1/100 - 1/200) IHC~~WB (1/1000 - 1/2000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	TUBA1B
Function	Tubulin is the major constituent of microtubules, protein filaments consisting of alpha- and beta-tubulin heterodimers (PubMed:38305685, PubMed:34996871, PubMed:38609661). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:38305685, PubMed:34996871, PubMed:38609661). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871, PubMed:38609661).
Cellular Location	Cytoplasm, cytoskeleton