Anti-ERCC1 Antibody

Mouse monoclonal antibody to ERCC1

Western blot analysis of ERCC1 expression in Hela (A), HepG2 (B), 293T (C), Jurkat (D) whole cell lysates.
Immunohistochemical analysis of ERCC1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Primary Accession	P07992
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	32562 Da

Additional Information
Gene ID	2067
Other Names	DNA excision repair protein ERCC-1
Target/Specificity	Recognizes endogenous levels of ERCC1 protein.
Dilution	WB~~WB (1/1000 - 1/2000), IHC (1/100 - 1/200) IHC~~WB (1/1000 - 1/2000), IHC (1/100 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Protein Information
Name	ERCC1
Function	[Isoform 1]: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.
Cellular Location	[Isoform 1]: Nucleus [Isoform 3]: Nucleus

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.