Anti-Phosphotyrosine Antibody

Mouse monoclonal antibody to Phosphotyrosine

Western blot analysis of Phosphotyrosine expression in Jurkat (A), 293T (B) whole cell lysates.
Immunohistochemical analysis of Phosphotyrosine staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC
Host	Mouse
Clonality	Monoclonal

Additional Information
Target/Specificity	Purified protein corresponding to Phosphotyrosine.
Dilution	WB~~WB (1/1000 - 1/2000), IHC (1/50 - 1/200) IHC~~WB (1/1000 - 1/2000), IHC (1/50 - 1/200)
Format	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.09% (W/V) sodium azide.
Storage	Store at -20 °C.Stable for 12 months from date of receipt

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.