ETV4 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

All lanes: Anti-ETV4 Antibody (C-term) at 1:1000 dilution + Rat lung lysate Lysates/proteins at 20 µg per lane. Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ASP1615) at 1/15000 dilution. Observed band size: 54 KDa Blocking/Dilution buffer: 5% NFDM/TBST.
Formalin-fixed and paraffin-embedded human brain tissue reacted with ETV4 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Flow cytometric analysis of widr cells using ETV4 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, FC, E
Primary Accession	P43268
Other Accession	P28322
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	53938 Da
Antigen Region	419-447 aa

Additional Information
Gene ID	2118
Other Names	ETS translocation variant 4, Adenovirus E1A enhancer-binding protein, E1A-F, Polyomavirus enhancer activator 3 homolog, Protein PEA3, ETV4, E1AF, PEA3
Target/Specificity	This ETV4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 419-447 amino acids from the C-terminal region of human ETV4.
Dilution	WB~~1:1000 IHC-P~~1:100~500 FC~~1:10~50 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.05% (V/V) Proclin 300. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	ETV4 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	ETV4
Synonyms	E1AF, PEA3
Function	Transcriptional activator (PubMed:19307308, PubMed:31552090). May play a role in keratinocyte differentiation (PubMed:31552090).
Cellular Location	Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00237}.
Tissue Location	Expressed in keratinocytes.