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>   首页   >   产品   >   一抗   >   癌症   >   HK1 (Hexokinase) Antibody (N-term)   

HK1 (Hexokinase) Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - HK1 (Hexokinase) Antibody (N-term) AP8141a
    Western blot analysis of HK1 (arrow) using HK1 Antibody (N-term) (Cat.#AP8141a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the HK1 gene (Lane 2) (Origene Technologies).
  • 1 - HK1 (Hexokinase) Antibody (N-term) AP8141a
    HK1 Antibody (L93) (Cat. #AP8141a) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the HK1 antibody detected the HK1 protein (arrow).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P-Leica, E
Primary Accession P19367
Reactivity Human, Rat, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 102486 Da
Antigen Region 78-108 aa
Additional Information
Gene ID 3098
Other Names Hexokinase-1, Brain form hexokinase, Hexokinase type I, HK I, HK1
Target/Specificity This HK1 (Hexokinase) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 78-108 amino acids from the N-terminal region of human HK1 (Hexokinase).
Dilution WB~~1:1000
IHC-P-Leica~~N/A
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsHK1 (Hexokinase) Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name HK1 (HGNC:4922)
Function Catalyzes the phosphorylation of various hexoses, such as D- glucose, D-glucosamine, D-fructose, D-mannose and 2-deoxy-D-glucose, to hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate and 2-deoxy-D-glucose 6- phosphate, respectively) (PubMed:1637300, PubMed:25316723, PubMed:27374331). Does not phosphorylate N-acetyl-D-glucosamine (PubMed:27374331). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (By similarity). Involved in innate immunity and inflammation by acting as a pattern recognition receptor for bacterial peptidoglycan (PubMed:27374331). When released in the cytosol, N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits the hexokinase activity of HK1 and causes its dissociation from mitochondrial outer membrane, thereby activating the NLRP3 inflammasome (PubMed:27374331).
Cellular Location Mitochondrion outer membrane; Peripheral membrane protein. Cytoplasm, cytosol. Note=The mitochondrial-binding peptide (MBP) region promotes association with the mitochondrial outer membrane (Probable). Dissociates from the mitochondrial outer membrane following inhibition by N-acetyl-D-glucosamine, leading to relocation to the cytosol (PubMed:27374331).
Tissue Location Isoform 2: Erythrocyte specific (Ref.6). Isoform 3: Testis-specific (PubMed:10978502). Isoform 4: Testis-specific (PubMed:10978502). {ECO:0000269|PubMed:10978502, ECO:0000269|Ref.6}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. The hexokinase gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of the hexokinase gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. HK1 encodes the ubiquitously expressed isoform. Its 5' end includes an exon which is unique to this transcript and which encodes a distinct N-terminus that contains the porin binding domain (PBD). The porin binding domain mediates association with the mitochondrial membrane.

REFERENCES

van Wijk, R., et al., Blood 101(1):345-347 (2003).
Murakami, K., et al., Acta Haematol. 108(4):204-209 (2002).
Murakami, K., et al., Mol. Genet. Metab. 67(2):118-130 (1999).
Aleshin, A.E., et al., Structure 6(1):39-50 (1998).
Ruzzo, A., et al., Blood 91(1):363-364 (1998).

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