MOSC1 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Western blot analysis of MOSC1 Antibody (Center) (Cat. #AP9754c) in HepG2 cell line lysates (35ug/lane). MOSC1 (arrow) was detected using the purified Pab.
MOSC1 Antibody (Center) (Cat. #AP9754c) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MOSC1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q5VT66
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	37499 Da
Antigen Region	175-204 aa

Additional Information
Gene ID	64757
Other Names	Mitochondrial amidoxime-reducing component 1, mARC1, 1---, Molybdenum cofactor sulfurase C-terminal domain-containing protein 1, MOSC domain-containing protein 1, Moco sulfurase C-terminal domain-containing protein 1, MARC1, MOSC1
Target/Specificity	This MOSC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 175-204 amino acids from the Central region of human MOSC1.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MOSC1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	MTARC1 (HGNC:26189)
Synonyms	MARC1, MOSC1
Function	Catalyzes the reduction of N-oxygenated molecules, acting as a counterpart of cytochrome P450 and flavin-containing monooxygenases in metabolic cycles (PubMed:19053771, PubMed:21029045, PubMed:30397129). As a component of prodrug-converting system, reduces a multitude of N-hydroxylated prodrugs particularly amidoximes, leading to increased drug bioavailability (PubMed:19053771). May be involved in mitochondrial N(omega)-hydroxy-L-arginine (NOHA) reduction, regulating endogenous nitric oxide levels and biosynthesis (PubMed:21029045). Postulated to cleave the N-OH bond of N-hydroxylated substrates in concert with electron transfer from NADH to cytochrome b5 reductase then to cytochrome b5, the ultimate electron donor that primes the active site for substrate reduction (PubMed:19053771, PubMed:21029045).
Cellular Location	Mitochondrion outer membrane; Single-pass type II membrane protein. Membrane; Lipid-anchor. Note=Mitochondrial import is mediated by AA 1-40 and requires ATP