WT1

Rabbit Monoclonal antibody(Mab)

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P
Primary Accession	P19544
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone Names	401B5E7
Calculated MW	49188 Da

Additional Information
Gene ID	7490
Gene Name	WT1
Other Names	Wilms tumor protein, WT33, WT1
Dilution	IHC-P~~Ready-to-use
Storage	Maintain refrigerated at 2-8°C.
Precautions	WT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	WT1
Function	Transcription factor that plays an important role in cellular development and cell survival (PubMed:7862533). Recognizes and binds to the DNA sequence 5'-GCG(T/G)GGGCG-3' (PubMed:17716689, PubMed:25258363, PubMed:7862533). Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors (PubMed:15520190). Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing (PubMed:16934801). Isoform 1 has lower affinity for DNA, and can bind RNA (PubMed:19123921).
Cellular Location	Nucleus. Nucleus, nucleolus. Cytoplasm. Note=Isoforms lacking the KTS motif have a diffuse nuclear location (PubMed:15520190). Shuttles between nucleus and cytoplasm. {ECO:0000250, ECO:0000269\|PubMed:15520190} [Isoform 4]: Nucleus, nucleoplasm
Tissue Location	Expressed in the kidney and a subset of hematopoietic cells