SDHA Antibody

Mouse Monoclonal Antibody (Mab)

Immunohistochemical analysis of paraffin-embedded colorectal carcinoma; tissue using AD80270 performed on the Abcarta® FAIP-48 Fully automated IHC platform.Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a Citrate buffer (pH6. 0).Samples were incubated with primary antibody(Ready-to-use) for 15 min at room temperature. AmpSeeTM Detection Systems（Abcepta:ADR005） was used as the secondary antibody.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC
Primary Accession	P31040
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Isotype	IgG
Clone Names	688B3D5
Calculated MW	72692 Da

Additional Information
Gene ID	6389
Gene Name	SDHA
Other Names	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial, 1.3.5.1, Flavoprotein subunit of complex II, Fp, SDHA, SDH2, SDHF
Dilution	IHC~~Ready-to-use
Storage	Maintain refrigerated at 2-8°C.
Precautions	SDHA Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SDHA
Synonyms	SDH2, SDHF
Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) (PubMed:10746566, PubMed:24781757). SDH also oxidizes malate to the non-canonical enol form of oxaloacetate, enol- oxaloacetate (By similarity). Enol-oxaloacetate, which is a potent inhibitor of the succinate dehydrogenase activity, is further isomerized into keto-oxaloacetate (By similarity). Can act as a tumor suppressor (PubMed:20484225).
Cellular Location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.