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C12orf30 Antibody (Center)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - C12orf30 Antibody (Center) AP10270C
    Anti-C12orf30 Antibody (Center) at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 112 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - C12orf30 Antibody (Center) AP10270C
    C12orf30 Antibody (Center) (Cat. #AP10270c) western blot analysis in mouse cerebellum tissue lysates (15ug/lane).This demonstrates the C12orf30 antibody detected C12orf30 protein (arrow).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q14CX7
Other Accession NP_079229.2
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 112292 Da
Additional info
Gene ID 80018
Other Names N-alpha-acetyltransferase 25, NatB auxiliary subunit, Mitochondrial distribution and morphology protein 20, N-terminal acetyltransferase B complex subunit MDM20, NatB complex subunit MDM20, N-terminal acetyltransferase B complex subunit NAA25, p120, NAA25, C12orf30, MDM20, NAP1
Target/Specificity This C12orf30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 720-748 amino acids from the Central region of human C12orf30.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsC12orf30 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name NAA25
Synonyms C12orf30, MDM20, NAP1
Function Non-catalytic subunit of the NatB complex which catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Asp-Glu. May play a role in normal cell-cycle progression.
Cellular Location Cytoplasm.
Research Areas
pTSara-NatB, an improved N-terminal acetylation system for recombinant protein expression in E. coli.
Author : Rovere M1,Powers AE1,Patel DS1,Bartels T1.
PLoS One. 2018 Jul 11;13(7):e0198715. doi: 10.1371/journal.pone.0198715. eCollection 2018.
29995905

BACKGROUND

This gene encodes the auxiliary subunit of the heteromeric N-terminal acetyltransferase B complex. This complex acetylates methionine residues that are followed by acidic or asparagine residues.

REFERENCES

Feng, T., et al. Hum. Genet. 128(3):269-280(2010)
Pierce, B.L., et al. Hum. Hered. 69(3):193-201(2010)
Douroudis, K., et al. BMC Med. Genet. 11, 11 (2010) :
Prahalad, S., et al. Arthritis Rheum. 60(7):2124-2130(2009)
Polevoda, B., et al. BMC Proc 3 SUPPL 6, S2 (2009) :

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