SHISA6 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

SHISA6 Antibody (N-term) (Cat. #AP11913a) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SHISA6 antibody detected the SHISA6 protein (arrow).
SHISA6 Antibody (N-term) (Cat. #AP11913a)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SHISA6 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q6ZSJ9
Other Accession	Q3UH99, NP_997269.2
Reactivity	Human
Predicted	Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	55764 Da
Antigen Region	81-109 aa

Additional Information
Gene ID	388336
Other Names	Protein shisa-6 homolog, SHISA6
Target/Specificity	This SHISA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-109 amino acids from the N-terminal region of human SHISA6.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	SHISA6 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	SHISA6 (HGNC:34491)
Function	Involved in maintenance of high-frequency synaptic transmission at hippocampal CA3-CA1 synapses. Regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression. May play a role in self-renewal and differentiation of spermatogonial stem cells by inhibiting canonical Wnt signaling pathway.
Cellular Location	Membrane; Single-pass type I membrane protein {ECO:0000250\|UniProtKB:Q3UH99}. Postsynaptic density {ECO:0000250\|UniProtKB:Q3UH99}
Tissue Location	Expressed in the developing ventral mesencephalon.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.