MBD3L3 Antibody (C-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-MBD3L3 Antibody (C-term) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 23 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
MBD3L3 Antibody (C-term) (Cat. #AP12132b)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBD3L3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Confocal immunofluorescent analysis of MBD3L3 Antibody (C-term)(Cat#AP12132b) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
MBD3L3 Antibody (C-term) (Cat. #AP12132b) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IHC-P, IF, FC, WB, E
Primary Accession	A6NE82
Other Accession	A6NJ08, A6NDZ8, Q8NHZ7, NP_001157897.1
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	23095 Da
Antigen Region	156-184 aa

Additional Information
Gene ID	653657
Other Names	Putative methyl-CpG-binding domain protein 3-like 3, MBD3-like protein 3, MBD3L3
Target/Specificity	This MBD3L3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 156-184 amino acids from the C-terminal region of human MBD3L3.
Dilution	IHC-P~~1:100~500 IF~~1:10~50 FC~~1:10~50 WB~~1:1000 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	MBD3L3 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.