癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
EIF1AY Antibody (N-term)
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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- 背景知识
Application 
| WB, IHC-P, E |
|---|---|
| Primary Accession | O14602 |
| Other Accession | Q6VV72, P47814, Q60872, Q8BMJ3, P47813, NP_004672.2 |
| Reactivity | Human |
| Predicted | Mouse, Rabbit, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 16442 Da |
| Antigen Region | 1-30 aa |
| Gene ID | 9086 |
|---|---|
| Other Names | Eukaryotic translation initiation factor 1A, Y-chromosomal, eIF-1A Y isoform, Eukaryotic translation initiation factor 4C, eIF-4C, EIF1AY |
| Target/Specificity | This EIF1AY antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human EIF1AY. |
| Dilution | WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | EIF1AY Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | EIF1AY |
|---|---|
| Function | Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. This protein enhances formation of the cap-proximal complex. Together with EIF1, facilitates scanning, start codon recognition, promotion of the assembly of 48S complex at the initiation codon (43S PIC becomes 48S PIC after the start codon is reached), and dissociation of aberrant complexes. After start codon location, together with EIF5B orients the initiator methionine-tRNA in a conformation that allows 60S ribosomal subunit joining to form the 80S initiation complex. Is released after 80S initiation complex formation, just after GTP hydrolysis by EIF5B, and before release of EIF5B. Its globular part is located in the A site of the 40S ribosomal subunit. Its interaction with EIF5 during scanning contribute to the maintenance of EIF1 within the open 43S PIC. In contrast to yeast orthologs, does not bind EIF1. |
| Cellular Location | Cytoplasm. |
| Tissue Location | Ubiquitous. |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
This gene encodes a protein similar to eukaryotic translation initiation factor 1A (EIF1A). EIF1A is required for the binding of the 43S complex (a 40S subunit, eIF2/GTP/Met-tRNAi and eIF3) to the 5' end of capped RNA.
REFERENCES
Serajee, F.J., et al. J. Child Neurol. 24(10):1258-1261(2009)
Lim, J., et al. Cell 125(4):801-814(2006)
Fortna, A., et al. PLoS Biol. 2 (7), E207 (2004) :
Agate, R.J., et al. Mol. Biol. Evol. 21(2):384-396(2004)
Skaletsky, H., et al. Nature 423(6942):825-837(2003)
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