C5orf33 Antibody (Center)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

C5orf33 Antibody (Center) (Cat. #AP13805c) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the C5orf33 antibody detected the C5orf33 protein (arrow).
C5orf33 Antibody (Center) (Cat. #AP13805c) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the C5orf33 antibody detected the C5orf33 protein (arrow).
C5orf33 Antibody (Center) (Cat. #AP13805c)immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of C5orf33 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q4G0N4
Other Accession	Q1HCL7, Q8C5H8, NP_694558.1, NP_001078880.1
Reactivity	Human, Mouse
Predicted	Rat
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	49433 Da
Antigen Region	122-150 aa

Additional Information
Gene ID	133686
Other Names	NAD kinase 2, mitochondrial, Mitochondrial NAD kinase, NAD kinase domain-containing protein 1, mitochondrial, NADK2, C5orf33, MNADK, NADKD1
Target/Specificity	This C5orf33 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122-150 amino acids from the Central region of human C5orf33.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	C5orf33 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	NADK2
Synonyms	C5orf33, MNADK, NADKD1
Function	Mitochondrial NAD(+) kinase that phosphorylates NAD(+) to yield NADP(+). Can use both ATP or inorganic polyphosphate as the phosphoryl donor. Also has weak NADH kinase activity in vitro; however NADH kinase activity is much weaker than the NAD(+) kinase activity and may not be relevant in vivo.
Cellular Location	Mitochondrion.
Tissue Location	Widely expressed..

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.