GDPD3 Antibody (N-term)

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Anti-GDPD3 Antibody (N-term) at 1:1000 dilution + MCF-7 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 36 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
GDPD3 Antibody (N-term) (Cat. #AP13964a) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GDPD3 antibody detected the GDPD3 protein (arrow).
GDPD3 Antibody (N-term) (Cat. #AP13964a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GDPD3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, E
Primary Accession	Q7L5L3
Other Accession	NP_077283.2
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Calculated MW	36596 Da
Antigen Region	17-46 aa

Additional Information
Gene ID	79153
Other Names	Glycerophosphodiester phosphodiesterase domain-containing protein 3, 31--, GDPD3
Target/Specificity	This GDPD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 17-46 amino acids from the N-terminal region of human GDPD3.
Dilution	WB~~1:1000 IHC-P~~1:100~500 E~~Use at an assay dependent concentration.
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	GDPD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Protein Information
Name	GDPD3 (HGNC:28638)
Function	Hydrolyzes lysoglycerophospholipids to produce lysophosphatidic acid (LPA) and the corresponding amines (PubMed:27637550). Shows a preference for 1-O-alkyl-sn-glycero-3- phosphocholine (lyso-PAF), lysophosphatidylcholine (lyso-PC) and N- acylethanolamine lysophospholipids (PubMed:27637550). Does not display glycerophosphodiester phosphodiesterase activity, since it cannot hydrolyze either glycerophosphoinositol or glycerophosphocholine.
Cellular Location	Membrane {ECO:0000250\|UniProtKB:Q99LY2}; Multi- pass membrane protein {ECO:0000250\|UniProtKB:Q99LY2}. Cytoplasm, perinuclear region {ECO:0000250\|UniProtKB:Q99LY2}. Endoplasmic reticulum. Note=Partially co-localized with CANX. {ECO:0000250\|UniProtKB:Q99LY2}
Tissue Location	Widely expressed, with high level in kidney and ovary.

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.