癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
SERBP1 Antibody (Center)
Purified Rabbit Polyclonal Antibody (Pab)
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- 实验流程
- 背景知识
Application 
| WB, E |
|---|---|
| Primary Accession | Q8NC51 |
| Reactivity | Human, Rat, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44965 Da |
| Gene ID | 26135 |
|---|---|
| Other Names | Plasminogen activator inhibitor 1 RNA-binding protein, PAI1 RNA-binding protein 1, PAI-RBP1, SERPINE1 mRNA-binding protein 1, SERBP1, PAIRBP1 |
| Target/Specificity | This SERBP1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 149-183 amino acids from the Central region of human SERBP1. |
| Dilution | WB~~1:1000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | SERBP1 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | SERBP1 {ECO:0000303|PubMed:36691768, ECO:0000312|HGNC:HGNC:17860} |
|---|---|
| Function | Ribosome-binding protein that promotes ribosome hibernation, a process during which ribosomes are stabilized in an inactive state and preserved from proteasomal degradation (PubMed:36691768). Acts via its association with EEF2/eEF2 factor, sequestering EEF2/eEF2 at the A- site of the ribosome and promoting ribosome stabilization and storage in an inactive state (By similarity). May also play a role in the regulation of mRNA stability: binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay (PubMed:11001948). Seems to play a role in PML-nuclear bodies formation (PubMed:28695742). |
| Cellular Location | Cytoplasm. Nucleus. Cytoplasm, perinuclear region |
| Tissue Location | Expressed at high level in the heart, skeletal muscle and kidney, and at low levels in placenta, liver and brain |
For Research Use Only. Not For Use In Diagnostic Procedures.
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
May play a role in the regulation of mRNA stability. Binds to the 3'-most 134 nt of the SERPINE1/PAI1 mRNA, a region which confers cyclic nucleotide regulation of message decay.
REFERENCES
Lai C.-H.,et al.Genome Res. 10:703-713(2000).
Wiemann S.,et al.Genome Res. 11:422-435(2001).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Gregory S.G.,et al.Nature 441:315-321(2006).
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Anonymous
2016-08-24 09:20:44
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Species tested
Human,Mouse
Application tested
WB
Organization tested
A549,HepG2,MCF-7,PC12,U87,B-cell,Liver(M),Thymus(M),Kindey(R)
Barcode encoding
Brief protocol
1. Block with 3% skim milk for 1 hour at room temperature.
2. Incubate overnight with Abgent primary antibody 1:1000 in 3% skim milk at 4℃
3. Wash 5*5 min with TBST.
4. Incubate with HRP-conjugated secondary antibody 1:5000 in 3% skim milk for 1 hour at room temperature.
5. Wash 5*5 min with TBST.
6. Incubate with ECL substrates and expose
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