癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Phospho-CREB(S133) Antibody
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- 产品详情
- 文献引用 : 1
- 实验流程
- 背景知识
Application 
| IHC-P-Leica, IF, WB, E |
|---|---|
| Primary Accession | P16220 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Calculated MW | 36688 Da |
| Gene ID | 1385 |
|---|---|
| Other Names | Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1 |
| Target/Specificity | This CREB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB. |
| Dilution | IHC-P-Leica~~1:500 WB~~1:500 |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is first purified by protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | Phospho-CREB(S133) Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CREB1 |
|---|---|
| Function | Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters. Transcription activation is enhanced by the TORC coactivators which act independently of Ser-133 phosphorylation. Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells. |
| Cellular Location | Nucleus {ECO:0000255|PROSITE- ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083} |
|
Author : Abe K1, Matsui S2, Watanabe D3. Proc Natl Acad Sci U S A. 2015 Jun 5. pii: 201413484. [Epub ahead of print] |
26048905 |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
REFERENCES
Samten, B., et al., J. Immunol. 174(10):6357-6363 (2005).
Zhang, X., et al., Proc. Natl. Acad. Sci. U.S.A. 102(12):4459-4464 (2005).
Cho, K.N., et al., J. Biol. Chem. 280(8):6676-6681 (2005).
Bailey, J., et al., J. Mol. Endocrinol. 34(1):1-17 (2005).
Perry, C., et al., Neoplasia 6(3):279-286 (2004).
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
