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Phospho-CREB(S133) Antibody

Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 59 - Phospho-CREB(S133) Antibody AP3077a
    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue using AP3077a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 59 - Phospho-CREB(S133) Antibody AP3077a
    Immunohistochemical analysis of paraffin-embedded Human colon carcinoma tissue using AP3077a performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.
  • 3 - Phospho-CREB(S133) Antibody AP3077a
    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells labeling CREB1 with AP3077a at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing Nucleus and Weak Cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin(red). The nuclear counter stain is DAPI (blue).
  • 1 - Phospho-CREB(S133) Antibody AP3077a
    Western blot analysis of lysates from Hela cell line, untreated or treated with TPA, 200nM, using Phospho-CREB(S133) Antibody(upper) or tubulin(lower).
  • 1 - Phospho-CREB(S133) Antibody AP3077a
    Western blot analysis of lysates from Hela cell line, untreated or treated with 20% FBS + 100nM Calyculin A, using Phospho-CREB(S133) Antibody(upper) or tubulin(lower).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, IHC-P-Leica, E
Primary Accession P16220
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 35136 Da
Additional Information
Gene ID 1385
Other Names Cyclic AMP-responsive element-binding protein 1, CREB-1, cAMP-responsive element-binding protein 1, CREB1
Target/Specificity This CREB Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB.
Dilution WB~~1:500
IHC-P~~N/A
IF~~1:25
IHC-P-Leica~~1:500
E~~Use at an assay dependent concentration.
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsPhospho-CREB(S133) Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CREB1
Function Phosphorylation-dependent transcription factor that stimulates transcription upon binding to the DNA cAMP response element (CRE), a sequence present in many viral and cellular promoters (By similarity). Transcription activation is enhanced by the TORC coactivators which act independently of Ser-119 phosphorylation (PubMed:14536081). Involved in different cellular processes including the synchronization of circadian rhythmicity and the differentiation of adipose cells (By similarity). Regulates the expression of apoptotic and inflammatory response factors in cardiomyocytes in response to ERFE-mediated activation of AKT signaling (By similarity).
Cellular Location Nucleus {ECO:0000255|PROSITE-ProRule:PRU00312, ECO:0000255|PROSITE-ProRule:PRU00978, ECO:0000269|PubMed:12552083}
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

BACKGROUND

CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.

REFERENCES

Samten, B., et al., J. Immunol. 174(10):6357-6363 (2005).
Zhang, X., et al., Proc. Natl. Acad. Sci. U.S.A. 102(12):4459-4464 (2005).
Cho, K.N., et al., J. Biol. Chem. 280(8):6676-6681 (2005).
Bailey, J., et al., J. Mol. Endocrinol. 34(1):1-17 (2005).
Perry, C., et al., Neoplasia 6(3):279-286 (2004).

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