Cytokeratin 6 Monoclonal Antibody(4A1)

Immunohistochemical analysis of paraffin-embedded Human-breast-cancer tissue. 1,CytokeRatin 6 Monoclonal Antibody(4A1) was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Human-breast-cancer tissue. 1,Cytokeratin 6 Monoclonal Antibody(4A1)(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Western blot analysis of 1) Hela, 2) HepG2, diluted at 1:2000.

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P, IF
Primary Accession	P02538, P04259, P48668
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Calculated MW	60045 Da

Additional Information
Gene ID	3853
Dilution	WB~~WB: 1:1000 IF 1:200 IHC 1:50-300 IHC-P~~N/A IF~~1:50~200
Format	PBS, pH 7.4, containing 0.09% (W/V) sodium azide as Preservative and 50% Glycerol.
Storage Conditions	-20℃

Protein Information
Name	KRT6A
Synonyms	K6A, KRT6D
Function	Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.
Tissue Location	Expressed in the corneal epithelium (at protein level).

Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

Application Protocols

Provided below are standard protocols that you may find useful for product applications.