Anti-Human Adiponectin Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Reactivity	Human
Host	Mouse
Clonality	Monoclonal

Additional Information
Preparation	Produced in Balb/c mice using highly pure recombinant Human Adiponectin as the immunizing antigen. This antibody was purified from cell culture by Protein A affinity chromatography.
WesternBlot	To detect Human Adiponectin by Western Blot analysis this antibody can be used at a concentration of 1.0-2.0 μg/ml. When used in conjunction with compatible secondary reagents the detection limit for recombinant Human Adiponectin is 0.5-1.0 ng/lane, under reducing conditions and 1.0-2.0 ng/lane, under non-reducing conditions.
Sandwich	In a sandwich ELISA (assuming 100μl/well), a concentration of 2.0-4.0 μg/ml of this antibody will detect at least 500 pg/ml of recombinant Human Adiponectin when used in conjunction with compatible detection reagents at a concentration of approximately 0.5-1.0 μg/ml.
Formulation	A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Human Adiponectin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.