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Anti-Human IGF-BP5 Antibody

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal

Additional Information
Preparation	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hIGF-BP5. Anti-Human IGF-BP5 specific antibody was purified by affinity chromatography employing immobilized hIGF-BP5 matrix.
WesternBlot	To detect hIGF-BP5 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-BP5 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich	To detect hIGF-BP5 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human IGF-BP5 (60-192BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hIGF-BP5.
Immunohistochemistry	This antibody stained U-2 OS and U-251 MG cells. The primary antibody was incubated at 2.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm. *Additional Immunostaining data available. Please contact Tech Support for information.
Formulation	A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Human IGF-BP5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.