Anti-Murine IL-2 Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal

Additional Information
Preparation	Produced in Sprague Dawley rats using highly pure (>98%) recombinant murine IL-2 as the immunizing antigen. This IgG2A_κ antibody was purified from cell culture by Protein A affinity chromatography.
WesternBlot	To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.
Sandwich	In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 32 pg/ml of recombinant murine IL-2 when used with BioGems' biotinylated antigen affinity purified anti-murine IL-2 (61-002BT) as the detection antibody at a concentration of at least 1.0-2.0 µg/ml.
Formulation	A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Murine IL-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.