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Anti-Murine JE/MCP-1 Antibody

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal

Additional Information
Preparation	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mJE(MCP-1). Anti-Murine JE(MCP-1) specific antibody was purified by affinity chromatography employing immobilized mJE (MCP-1) matrix.
WesternBlot	To detect mJE(MCP-1) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mJE(MCP-1) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich	To detect mJE(MCP-1) by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Murine JE(MCP-1)(61-083BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mJE(MCP-1).
Immunohistochemistry	This antibody stained colchicine injected mouse brain (including caudate putamen) tissue. The primary antibody was incubated at 1.0 mg/ml overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent. Optimal concentrations and conditions may vary.
Neutralization	To yield one-half maximal inhibition [ND₅₀] of the biological activity of mJE(MCP-1) (100 ng/ml), a concentration of 4.0-6.0 µg/ml of this antibody is required.
Formulation	A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Murine JE/MCP-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.