Anti-Human LEC Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Reactivity	Human
Host	Mouse
Clonality	Monoclonal

Additional Information
Preparation	Produced in BALB/c x ICR F₁ mice using highly pure (>98%) recombinant human LEC as the immunizing antigen. This IgG1_K antibody was purified from ascites fluid by Protein G affinity chromatography.
WesternBlot	To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Sandwich	In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human LEC when used with BioGems' biotinylated antigen affinity purified anti-human LEC (60-203BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/ml.
Immunohistochemistry	To yield one-half maximal inhibition [ND₅₀] of the biological activity of Human LEC (100 ng/ml), a concentration of 1.0-5.0 μg/ml of this antibody is required.
Formulation	A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Human LEC Antibody is for research use only and not for use in diagnostic or therapeutic procedures.