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>   首页   >   产品   >   一抗   >   其他   >   Biotinylated Anti-Human NT-4 Antibody   

Biotinylated Anti-Human NT-4 Antibody

     
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Reactivity Human
Host Goat
Clonality Polyclonal
Additional Information
Preparation Produced from sera of goats pre-immunized with highly pure (>98%) recombinant hNT-4. Anti-Human NT-4 specific antibody was purified by affinity chromatography and then biotinylated.
WesternBlot To detect hNT-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNT-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich To detect hNT-4 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with BioGems' Polyclonal Anti-Human NT-4 (60-253P) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNT-4.
Direct To detect hNT-4 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hNT-4.
Formulation A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution Centrifuge vial prior to opening. Reconstitute in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Storage-20°C
PrecautionsBiotinylated Anti-Human NT-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Research Areas

For Research Use Only. Not For Use In Diagnostic Procedures.

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