Anti-Human SCF Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Reactivity	Human
Host	Mouse
Clonality	Monoclonal

Additional Information
Preparation	Produced in BALB/c x ICR F₁ mice using highly pure (>98%) recombinant human SCF as the immunizing antigen. This IgG1_K antibody was purified from ascites fluid by Protein A affinity chromatography.
WesternBlot	To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 2.0-4.0 ng/lane, under non-reducing conditions.
Sandwich	In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant human SCF when used with BioGems' biotinylated antigen affinity purified anti-human SCF (60-291BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
Immunohistochemistry	This antibody stained CACO-2 cells and A-431cells. The primary antibody was incubated at 2.0 µg/ml overnight at 4˚C followed by a fluorescent labeled secondary antibody. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.
Formulation	A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Human SCF Antibody is for research use only and not for use in diagnostic or therapeutic procedures.