Anti-Human sTNF Receptor Type I Antibody

Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, E
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal

Additional Information
Preparation	Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hsTNF-receptor. Anti-Human sTNF-receptor specific antibody was purified by affinity chromatography employing immobilized hsTNF-receptor matrix.
WesternBlot	To detect hsTNF-receptor by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich	To detect hsTNF-receptor by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human sTNF-receptor (60-327BT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hsTNF-receptor.
Neutralization	To yield one-half maximal inhibition [ND₅₀] of the biological activity of hsTNF-receptor(0.3 µg/ml), a concentration of 0.9-1.1 µg/ml of this antibody is required.
Formulation	A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Human sTNF Receptor Type I Antibody is for research use only and not for use in diagnostic or therapeutic procedures.