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Anti-Human TNF-α Antibody

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Reactivity	Human
Host	Goat
Clonality	Polyclonal

Additional Information
Preparation	Produced from sera of goats pre-immunized with highly pure recombinant Human TNF-α. Anti-Human TNF-α specific antibody was purified by affinity chromatography employing immobilized Human TNF-α matrix.
WesternBlot	To detect Human TNF-α by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Human TNF-α is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Sandwich	To detect Human TNF-α by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with BioGems' Biotinylated Anti-Human TNF-α (60-325GBT) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human TNF-α.
Immunohistochemistry	This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentrations are 2.5 μg/ml-5.0 μg/ml with a two-hour incubation at room temperature or an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. This antibody was also tested in an HRP-biotin labeling detection system. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Optimal concentrations and conditions may vary.
Neutralization	To yield one-half maximal inhibition [ND₅₀] of the biological activity of Human TNF-α (0.25 ng/ml), a concentration of 0.012 - 0.014 µg/ml of this antibody is required.
Formulation	A sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Human TNF-α Antibody is for research use only and not for use in diagnostic or therapeutic procedures.