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Anti-Human sTRAIL/Apo2L Antibody

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC, E
Reactivity	Human
Host	Mouse
Clonality	Monoclonal

Additional Information
Preparation	Produced in BALB/c x ICR F₁ mice using highly pure (>98%) recombinant human TRAIL/APO2L as the immunizing antigen. This IgG1_K antibody was purified from ascites fluid by Protein A affinity chromatography.
WesternBlot	To detect Human sTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human sTRAIL/Apo2L is 0.25-1.0 ng/lane, under reducing or non-reducing conditions.
Sandwich	In a sandwich ELISA (assuming 100µl/well), a concentration of 2.0-4.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant human sTRAIL/APO2L when used with BioGems' biotinylated antigen affinity purified anti-human sTRAIL/APO2L (60-330BT) as the detection antibody at a concentration of approximately 1.0-2.0 µg/ml.
Immunohistochemistry	This antibody stained formalin-fixed, paraffin-embedded sections of human renal cell carcinoma. The recommended concentration is 2.5 µg/mL- 1.5 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 9.0 buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Information and photo are courtesy of Flagship Biosciences, LLC – Histology Services.
Neutralization	To yield one-half maximal inhibition [ND₅₀] of the biological activity of Human TRAIL/Apo2L (10.0 ng/ml), a concentration of 0.30-0.60 μg/ml of this antibody is required.
Formulation	A sterile filtered antibody solution was lyophilized from PBS.
Reconstitution	Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml.
Storage	-20°C
Precautions	Anti-Human sTRAIL/Apo2L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.