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Anti-Sca1/Ly6A/E Picoband Antibody

Figure 1. Western blot analysis of Sca1/Ly6A/E using anti- Sca1/Ly6A/E antibody (ABO10354). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Sca1/Ly6A/E antigen affinity purified polyclonal antibody (Catalog # ABO10354) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sca1/Ly6A/E at approximately 17KD. The expected band size for Sca1/Ly6A/E is at 14KD.

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Product Information
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB
Primary Accession	P05533
Host	Rabbit
Reactivity	Mouse
Clonality	Polyclonal
Format	Lyophilized
Description	Rabbit IgG polyclonal antibody for Lymphocyte antigen 6A-2/6E-1(Ly6a) detection. Tested with WB in Mouse.
Reconstitution	Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Additional Information
Gene ID	110454
Other Names	Lymphocyte antigen 6A-2/6E-1, Ly-6A.2/Ly-6E.1, Stem cell antigen 1, SCA-1, T-cell-activating protein, TAP, Ly6a, Ly6
Calculated MW	14377 Da
Application Details	Western blot, 0.1-0.5 µg/ml, Mouse
Subcellular Localization	Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Specificity	Widely expressed. .
Source	Eukaryota
Protein Name	Lymphocyte antigen 6A-2/6E-1
Contents	Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen	A synthetic peptide corresponding to a sequence in the middle region of mouse Sca1/Ly6A/E (48-75aa YPDGVCVTQEAAVIVDSQTRKVKNNLCL).
Purification	Immunogen affinity purified.
Cross Reactivity	No cross reactivity with other proteins.
Storage	At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.

For Research Use Only. Not For Use In Diagnostic Procedures.

Protein Information
Name	Ly6a
Synonyms	Ly6
Function	T-cell activation.
Cellular Location	Cell membrane; Lipid-anchor, GPI-anchor.
Tissue Location	Widely expressed.

Research Areas

Application Protocols

Provided below are standard protocols that you may find useful for product applications.

Western Blot Protocol

Blocking Peptides Protocol

Dot Blot Protocol

Immunohistochemistry Protocol

Immunofluorescence Protocol

Immunoprecipitation Protocol

Flow Cytomety Protocol

Cell Culture Protocol

BACKGROUND

Stem cell antigen-1 (Sca-1) is a mouse glycosyl phosphatidylinositol-anchored protein and a cell surface marker found on hematopoietic stem cells (HSCs). It is encoded by the strain-specific Ly-6 E/A allelic gene. Sca-1 has been discovered in several non hematopoietic tissues and can be used to enrich progenitor cell populations other than HSC. It is suggested that Sca-1 could be involved in regulating both B and T cell activation.

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¥ 3,367.00

Cat# ABO10354

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100 µg

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¥ 3,367.00

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Anti-Sca1/Ly6A/E Picoband Antibody

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