癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-Bcl-2 Antibody (Monoclonal, Bcl-2-100)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, IHC-P, IHC-F, ICC |
|---|---|
| Primary Accession | P49950 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2 (BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 24224 |
|---|---|
| Other Names | Apoptosis regulator Bcl-2, Bcl2, Bcl-2 |
| Calculated MW | 26622 Da |
| Application Details | Immunohistochemistry(Paraffin-embedded Section), 0.4-1 µg/ml, Human, By Heat Immunocytochemistry , 1 µg/ml, Human, - Immunohistochemistry(Frozen Section), 0.4-1 µg/ml, Human, - Western blot, 1-2 µg/ml, Human |
| Subcellular Localization | Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. |
| Tissue Specificity | Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate. |
| Source | Eukaryota |
| Protein Name | Apoptosis regulator Bcl-2 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | Bcl-2-100 |
| Immunogen | Synthetic peptide corresponding to residues 41-54 of the bcl-2 protein, conjugated to thyroglobulin. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the Bcl-2 family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Bcl2 |
|---|---|
| Synonyms | Bcl-2 |
| Function | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function. May attenuate inflammation by impairing NLRP1- inflammasome activation, hence CASP1 activation and IL1B release. |
| Cellular Location | Mitochondrion outer membrane {ECO:0000250|UniProtKB:P10415}; Single-pass membrane protein. Nucleus membrane {ECO:0000250|UniProtKB:P10415}; Single- pass membrane protein. Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:P10415}; Single-pass membrane protein. Cytoplasm {ECO:0000250|UniProtKB:P10417} |
| Tissue Location | Expressed in a variety of tissues, with highest levels in reproductive tissues. In the adult brain, expression is localized in mitral cells of the olfactory bulb, granule and pyramidal neurons of hippocampus, pontine nuclei, cerebellar granule neurons, and in ependymal cells. In prenatal brain, expression is higher and localized in the neuroepithelium and in the cortical plate |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of BCL2 protein, regardless of whether the breakpoint was located in or at a distance from the BCL2 gene. Overexpression of BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
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