癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-CD8 Antibody (Monoclonal, CA-8)
- 产品详情
- 实验流程
- 背景知识
Application 
| IHC-F |
|---|---|
| Primary Accession | P05541 |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for CD8, CD8a molecule; CD8b molecule (CD8A; CD8B) detection. Tested with IHC-F in Human. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 24931 |
|---|---|
| Other Names | T-cell surface glycoprotein CD8 beta chain, CD8 antigen 37 kDa chain, OX-8 membrane antigen, CD8b, Cd8b, Cd8b1 |
| Calculated MW | 23400 Da |
| Application Details | Immunohistochemistry(Frozen Section), 1 µg/ml, Human |
| Subcellular Localization | Membrane; Single-pass type I membrane protein. |
| Source | Eukaryota |
| Protein Name | T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | CA-8 |
| Immunogen | Human thymocytes followed by peripheral blood T cells. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Contains 1 Ig-like V-type (immunoglobulin-like) domain. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Cd8b |
|---|---|
| Synonyms | Cd8b1 |
| Function | Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide complex. The antigens presented by class I peptides are derived from cytosolic proteins while class II derived from extracellular proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class I proteins presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. A palmitoylation site in the cytoplasmic tail of CD8B chain contributes to partitioning of CD8 into the plasma membrane lipid rafts where signaling proteins are enriched. Once LCK recruited, it initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of cytotoxic T-lymphocytes (CTLs). Additionally, plays a critical role in thymic selection of CD8+ T- cells. |
| Cellular Location | Cell membrane {ECO:0000250|UniProtKB:P10966}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:P10966} Note=Requires the partner CD8A for efficient cell surface expression The heterodimer CD8A/CD8B localizes to lipid rafts due to CD8B cytoplasmic tail palmitoylation. {ECO:0000250|UniProtKB:P10966} |
Research Areas
Application Protocols
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
The human lymphocyte differentiation antigen CD8 is encoded by a single gene that gives rise to a 33- to 34-kDa glycoprotein expressed on the cell surface as a dimer and in higher molecular mass forms.
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