癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-CDC6 Antibody (Monoclonal, DCS-180)
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- 实验流程
- 背景知识
Application 
| WB |
|---|---|
| Primary Accession | O89033 |
| Host | Mouse |
| Isotype | Mouse IgG1 |
| Reactivity | Human |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for CDC6, cell division cycle 6 homolog (S. cerevisiae) (CDC6) detection. Tested with WB in Human. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 23834 |
|---|---|
| Other Names | Cell division control protein 6 homolog, CDC6-related protein, p62(cdc6), Cdc6 |
| Calculated MW | 62614 Da |
| Application Details | Western blot, 1-2 µg/ml, Human |
| Subcellular Localization | Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells. . |
| Source | Eukaryota |
| Protein Name | Cell division control protein 6 homolog |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | DCS-180 |
| Immunogen | Recombinant human Cdc6. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the CDC6/cdc18 family. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | Cdc6 |
|---|---|
| Function | Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated. |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:Q99741}. Cytoplasm {ECO:0000250|UniProtKB:Q99741}. Note=The protein is nuclear in G1 and cytoplasmic in S-phase cells. {ECO:0000250|UniProtKB:Q99741} |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
In yeasts, Cdc6(Saccharomyces cerevisiae) and Cdc18(Schizosaccharomyces pombe) associate with the origin recognition complex(ORC) proteins to render cells competent for DNA replication. Cdc6 is overexpressed in human cancers, where it has a critical regulatory role in addition to DNA replication. Yan et al.(1998) showed that Cdc6 is expressed selectively in proliferating but not quiescent mammalian cells, both in culture and within tissues in intact animals. During the transition from a growth-arrested to a proliferative state, transcription of mammalian Cdc6 is regulated by E2F proteins, as revealed by a functional analysis of the human Cdc6 promoter and by the ability of exogenously expressed E2F proteins to stimulate the endogenous Cdc6 gene. They conclude that expression of human Cdc6 is regulated in response to mitogenic signals though transcriptional control mechanisms involving E2F proteins, and that Cdc6 is required for initiation of DNA replication in mammalian cells.
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