癌症的基本特征包括细胞增殖、血管生成、迁移、凋亡逃避机制和细胞永生等。找到癌症发生过程中这些通路的关键标记物和对应的抗体用于检测至关重要。
为您推荐一个泛素化位点预测神器——泛素化分析工具，可以为您的蛋白的泛素化位点作出预测和评分。
细胞自噬受体图形绘图工具为你的蛋白的细胞受体结合位点作出预测和评分，识别结合到自噬通路中的蛋白是非常重要的，便于让我们理解自噬在正常生理、病理过程中的作用，如发育、细胞分化、神经退化性疾病、压力条件下、感染和癌症。
Anti-CDK4 Antibody (Monoclonal, DCS-31)
- 产品详情
- 实验流程
- 背景知识
Application 
| WB, ICC |
|---|---|
| Primary Accession | P35462 |
| Host | Mouse |
| Isotype | Mouse IgG2a |
| Reactivity | Human, Mouse, Rat |
| Clonality | Monoclonal |
| Format | Lyophilized |
| Description | Mouse IgG monoclonal antibody for CDK4, cyclin-dependent kinase 4 (CDK4) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins. |
| Reconstitution | Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Gene ID | 1814 |
|---|---|
| Other Names | D(3) dopamine receptor, Dopamine D3 receptor, DRD3 |
| Calculated MW | 44195 Da |
| Application Details | Immunocytochemistry , 1 µg/ml, Human, mouse, rat, - Western blot, 0.5-1 µg/ml, Human, mouse, rat |
| Subcellular Localization | Cell membrane ; Multi-pass membrane protein . Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co- localize in lipid rafts of renal proximal tubule cells. |
| Tissue Specificity | Brain. |
| Source | Eukaryota |
| Protein Name | Cyclin-dependent kinase 4 |
| Contents | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. |
| Clone Names | DCS-31 |
| Immunogen | Recombinant human Cdk4 protein. |
| Purification | Ascites |
| Cross Reactivity | No cross reactivity with other proteins |
| Storage | At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing. |
| Sequence Similarities | Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | DRD3 (HGNC:3024) |
|---|---|
| Function | Dopamine receptor that is primarily expressed in limbic areas of the brain and is involved in the modulation of cognitive, emotional, and endocrine functions (PubMed:39984436). Plays a key role in regulating neuronal signaling pathways associated with motivation, reward, and behavior (PubMed:39984436). Coupled to G(i)/G(o) proteins; activation leads to inhibition of adenylate cyclase and decreased intracellular cAMP levels (PubMed:10578130). Involved in the control of locomotor activity and implicated in several neuropsychiatric disorders, including schizophrenia and substance use disorders (PubMed:39984436). Promotes cell proliferation through MAP kinase signaling (PubMed:19520868). Also involved in autophagy regulation: receptor activation stimulates AMPK, which phosphorylates RPTOR and enhances its interaction with MTOR, thereby inhibiting MTORC1 signaling and its downstream target RPS6KB1. This leads to activation of ULK1 and initiation of the autophagy cascade (PubMed:31538542). Forms heterotetramers with DRD1 to potentiate beta-arrestin recruitment and mediate locomotor activity (By similarity). |
| Cellular Location | Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells |
| Tissue Location | Brain. |
Provided below are standard protocols that you may find useful for product applications.
BACKGROUND
Cyclin-dependent kinase-4(CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.
终于等到您。ABCEPTA（百远生物）抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息，您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。
如有疑问，联系：0512-88856768 tech-china@abcepta.com.
